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Research Interests
Research interests are focused on (molecular) biology and enzymology
of biotechnologically important filamentous fungi, in particular Trichoderma reesei. T. reesei
offers a powerful system for efficient expression of foreign gene
products originating from other micro-organisms, plants and animals.
Other research interests include biological roles of cell walls in
fungi and molecular prospecting of the environment for novel
bioactivities.
The research topics are:
- Development of vectors and hosts for efficient expression and secretion of gene products from T. reesei. Expression of thermophile enzyme genes in Trichoderma.
- Proteomes of Trichoderma. Mapping of cell wall-associated proteins in Trichoderma by 2-D electrophoresis. Emphasis is on the identification of proteins related to secretion and heterologous gene expression.
- Molecular prospecting of the environment. Screening of novel enzyme
activities and their genes from Antarctic fungi and unculturable
microorganisms.
Selected Publications
Professor Nevalainen has over 120 publications in refereed journals; 50 of which were published in the last 5 years. The ten most significant publications of the last 5 years are given below:
- B. R. Herbert, J. Grinyer, J. T. McCarthy, M. Isaacs, E. J. Harry, H. Nevalainen, M. D. Traini, B. Schulz, M. Laver, A. R. Goodall, J. Packer, J. L. Harry and K.L. Williams. Improved 2-DE of micro-organisms after acidic extraction. Electrophoresis 27: 1630-1640, 2006.
- K. M. H. Nevalainen V. S. J. T'eo and P. L. Bergquist. Heterologous protein expression in filamentous fungi. Trends in Biotechnology 23: 468-474, 2005.
- M. Penttilä, C. Limon and H. Nevalainen. Biotechnology of Trichoderma . Handbook of Fungal Biotechnology (Mycology Series 20), D.K. Arora, P.D. Bridge, D. Bhatnagar (eds), Marcel Dekker, NY, pp. 413-427, 2004 .
- H. Nevalainen, V. Te'o, T. Pakula and M. Penttilä. Heterologous gene expression in filamentous fungi-a holistic view. In : Applied Mycology & Biotechnology. An International Series. Volume 5, Genes and Genomics. D.K. Arora and G.G. Kchachatourians (eds), Elsevier Science B.V., Netherlands . pp. 211-237, 2005.
- J. Grinyer, M. McKay, H. Nevalainen and Ben R. Herbert (2004). Fungal Proteomics: initial mapping of biological control strain Trichoderma harzianum . Current Genetics 45: 163-169.
- J. Grinyer, M. McKay, B. Herbert and H. Nevalainen. Fungal Proteomics: mapping the mitochondrial proteins of a Trichoderma harzianum strain applied for biological control. Current Genetics 45:170-175, 2004.
- A. Khan, K. Williams, M. Molloy and H. Nevalainen. Purification and characterization of a serine protease and chitinases from Paecilomyces lilacinus and detection of chitinase Activity on 2D gels. Protein Expression and Purification 32: 210-220, 2003.
- P. Bergquist, V. Te'o, M. Gibbs, A. Cziferszky, F. P. de Faria, M. Azevedo and H. Nevalainen . Expression of
- D. Lim, P. Hains, B. Walsh, P. Bergquist and H. Nevalainen. Proteins associated with the cell envelope of Trichoderma reesei : a proteomic approach. Proteomics 1: 899 - 910, 2001.
- M. D. Gibbs K. H. M. Nevalainen and P. L. Bergquist. Degenerate oligonucleotide gene shuffling (DOGS): A method for enhancing the frequency of recombination with family shuffling. Gene 271: 13 – 20, 2001.
Competitive Grant Funding in last 5 years:
| Grant Source |
Applicant |
Title |
Years |
K$ |
| ARC Discovery |
Nevalainen et al |
DP0557601 Role of proteasome in eradication… |
2005-7 |
215 |
| ARCRes Networks |
Goldys et al |
RN0460002 Fluorescence applications in... |
2005-9 |
1800 |
| ARC LIEF |
Goldys et al |
LE0453608 Multifunctional confocal laser… |
2003 |
579 |
| ARC Linkage |
Nevalainen et al |
LP0349278 Enhanced biocontrol options… |
2003-5 |
230 |
| ARCSpecRes Initit |
Halbert et al |
SR0354630Fluorescenceapplicationsin… |
2003 |
20 |
| ARC Linkage |
Bergquist et al |
LP0347861 Genetic modification… |
2003-5 |
190 |
| ARC Linkage |
Bergquist et al |
LP0215935 Enhanced biocatalysis... |
2002-3 |
270 |
| ARC Discovery |
Gillings et al |
DP0211559 Bacterial innovation... |
2002-4 |
231 |
| ARC LIEF |
Scott et al |
LE0232455 The molecular analysis… |
2002 |
545 |
| ARC RIEF |
Trent et al |
R00107920 Sydney Gene Analysis Facility… |
2001 |
515 |
| ARC SPIRT |
Nevalainen et al |
C00107189 Heat-stable dextranase… |
2001-3 |
217 |
| ARC SPIRT |
Bergquist et al |
C00107398 Thermophilic DNA polym… |
2001-3 |
225 |
| ARC Large |
Nevalainen et al |
A00105749 What cell envelope… |
2001-3 |
181 |
| Res. Contracts |
Nevalainen et al |
With European companies on fungi |
2001-6 |
200 |
Contact Details
Links to Other pages
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Professor
Qualifications
BSc (Genetics)
University of Helsinki
MSc (Genetics)
University of Helsinki
PhD (Genetics)
University of Helsinki
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